Search results for "Crypthecodinium cohnii"
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Crypthecodinium cohnii Growth and Omega Fatty Acid Production in Mediums Supplemented with Extract from Recycled Biomass
2022
Crypthecodinium cohnii is a marine heterotrophic dinoflagellate that can accumulate high amounts of omega-3 polyunsaturated fatty acids (PUFAs), and thus has the potential to replace conventional PUFAs production with eco-friendlier technology. So far, C. cohnii cultivation has been mainly carried out with the use of yeast extract (YE) as a nitrogen source. In the present study, alternative carbon and nitrogen sources were studied: the extraction ethanol (EE), remaining after lipid extraction, as a carbon source, and dinoflagellate extract (DE) from recycled algae biomass C. cohnii as a source of carbon, nitrogen, and vitamins. In mediums with glucose and DE, the highest specific biomass gr…
Crypthecodinium cohnii metabolisma matemātiskā modelēšana DHA ražošanas optimizācijai
2020
Datormodelēšana un bioķīmisko ceļu simulēšana ir sistēmbioloģijas pamatnostādnes, un pastāv vairākas metodes, kas var palīdzēt šo sistēmu izpētīšanā. Darbā izmantota divu sistēmbioloģijas modelēšanas metožu pieeja šūnu taukskābju sintēzes metabolisma pētīšanai. Darba ietvaros izveidots kinētiskais modelis, kas ietver glikolīzes ceļa reakcijas un reakcijas, kas ved līdz taukskābju sintezējošajam enzīmam- FAS, kā arī pilnveidots genoma izmēra metaboliskais modelis dinoflagellātam Crypthecodinium cohnii. Kinētiskais modelis tika parametrizēts saskaņā ar pieejamajiem eksperimentālajiem datiem. Aplūkoti stehiometriskajā modelī ietvertie metabolisma regulācijas mehānismi taukskābju sintēzei un ga…
Syntrophy of Crypthecodinium cohnii and immobilized Zymomonas mobilis for docosahexaenoic acid production from sucrose-containing substrates
2021
Marine heterotrophic dinoflagellate Crypthecodinium cohnii is an aerobic oleaginous microorganism that accumulates intracellular lipid with high content of 4,7,10,13,16,19-docosahexaenoic acid (DHA), a polyunsaturated ω-3 (22:6) fatty acid with multiple health benefits. C. cohnii can grow on glucose and ethanol, but not on sucrose or fructose. For conversion of sucrose-containing renewables to C. cohnii DHA, we investigated a syntrophic process, involving immobilized cells of ethanologenic bacterium Zymomonas mobilis for fermenting sucrose to ethanol. The non-respiring, NADH dehydrogenase-deficient Z. mobilis strain Zm6-ndh, with high ethanol yield both under anaerobic and aerobic condition…